Scope of the 3 rd Summer School in Proteomic Basics

Proteomics has become an indispensable tool in molecular and cellular biology and for the emerging field of systems biology. On account of the diversities in protein sequence and structure, proteomics provides several different approaches and techniques for the investigation of proteins in their cellular environment.

The scope of this summer school is to give an overview of basic proteomic techniques for young scientists who have already started using such techniques or who intend to use proteomic approaches in their research.

It is increasingly important for young scientists to be familiar with the theoretical basics and the established and potential applications of the most common technologies within proteomics, if they wish to exploit the full the possibilities that these techniques offer.

Earlier Summer Schools in Proteomic Basics, which were held in Opatija (Croatia) in 2004 and in Brixen/Bressanone (Italy) in 2005, were a great success and gave a lot of positive feedback.

We are therefore pleased to announce the 3rd Summer School in Proteomic Basics "Exploring the Diversity of Proteins", taking place again in Brixen/Bressanone, South Tyrol, Italy from August 13 to August 19, 2006.

As in previous years, the Summer School is generously supported by the German Federal Ministry of Education and Science (BMBF), the German Society of Proteome Research (DGPF), and the Society of Biochemistry and Molecular Biology (GBM).

In addition to the tutorial lecture on basic proteomic techniques (which will be held by outstanding experts in the respective fields) we will also offer group meetings to discuss selected topics in depth on the basis of concrete examples taken from practical research situations.

Following topics will be covered by tutorial lectures:

  1. Importance of pre-fractionation and sample preparation
  2. One-, two- and multi-dimensional chromatography
  3. Two-dimensional gel electrophoresis
  4. Mass spectrometry techniques
  5. Membrane proteins
  6. Detection of post-translational modifications
  7. Quantification methods
  8. Protein complexes and protein interactions
  9. Bioinformatics

Group meetings will revolve around similar topics, i.e.:

  • Chromatography
  • Two-dimensional gel electrophoresis (including DIGE)
  • Mass spectrometry (MALDI vs. ESI)
  • MS-based detection of post-translational modifications (with emphasis on protein phosphorylation)
  • MS-based quantification approaches
  • MS-data evaluation, search engines, false positives

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